The metagenomic sequence data of the two cattle breeds viz., Kankrej, Gir and three buffalo breeds viz., Mehsani, Jaffarabadi, and Surti rumen samples were analyzed using MGRASTfor its microbial diversity and functional content. The role of methanogens in the rumen of Indian cattle and its comparative context with exotic cattle was performed. Rumen Microbiome analysis performed from animals raised at different locations i.e. SK Nagar and Anand revealed that the microbiome profile of the Mehsani buffalo and Kankrej Cows raised at SK Nagar was found to have higher similarity than Gir cows raised at Anand suggesting the higher influence of geographical location than the effect of species in shaping the microbial community. Preliminary data on the comparative microbiome profile suggested a predominance of Bacteoidetes in Mehsani buffalo and Kankrej Cows whereas of Firmicutes in Gir Cows. The study also generated basic information for the understanding of the complexity of the rumen microbial ecology with special attention to the resistome, Phages, Prophages, Transposable elements, Plasmids, and stress responses. Metatranscriptome sequencing runs were performed for all 48 samples of each breed with the aim to characterize the active rumen microbiome of cattle and buffaloes adapted to different diet treatments. 16S amplicon sequencing of all the breeds was completed and primer-wise, as well as sample-wise comparative analysis, was performed. All the data of metagenome and metatranscriptome sequencing were quality filtered at uniform parameters and uploaded on MGRAST for taxonomic and functional assessment. As per the proposed objective, collection of all the samples for the calf to adult stages was completed, DNA isolated, and amplicons generated from the same. Overall, the objectives of the program were completed with salient achievements under each technical program. The present study generated the basic information which delivers the understanding of the complexity of the microbial ecology of the cattle rumen with special attention to the resistome, Phages, Prophages, Transposable elements, Plasmids, and stress responses
Read More: http://www.aau.in/sites/default/files/Niche%20Area%20Project%20Report.pdf
